Plasmodium falciparum UvrD Helicase Translocates in 3′ to 5′ Direction, Colocalizes with MLH and Modulates Its Activity through Physical Interaction
Figure 3
Purification and ATPase activity analysis.
A, Silver-stained gel of purified PfUDN. Lane M contains the protein molecular weight marker and lane 1 and 2 contain 0.3 and 0.2 µg of the purified PfUDN. B, Silver-stained gel of purified PfUDC1. Lane M contains the protein molecular weight marker and lane 1 contains 0.2 µg of the purified PfUDC1. C, Silver-stained gel of purified PfUDC2. Lane M contains the protein molecular weight marker and lane 1 contains 0.2 µg of the purified PfUDC2. D, Western blot of purified PfUDN. Lane M contains the protein molecular weight marker and lane 1 and 2 contain 0.3 and 0.2 µg of the purified PfUDN. E, Western blot of purified PfUDC1. Lane M contains the protein molecular weight marker and lane 1 contains 0.2 µg of the purified PfUDC1. F, Western blot of purified PfUDC2. Lane M contains the protein molecular weight marker and lane 1 contains 0.2 µg of the purified PfUDC2. G, ATPase activity of purified PfUDN. Lane C, reaction without enzyme, Lanes 1–4, reactions with enzyme in the presence of ssDNA and Mg2+. H, The quantitative data of the autoradiogram in G. I, Time dependence of ATPase activity of PfUDN. The time of incubation in minutes is mentioned at the top of the autoradiogram and C is the control reaction without enzyme. J, The quantitative data of the autoradiogram in I. K, ATPase activity of purified PfUDC1. Lane C, reaction without enzyme, Lanes 1–4, reactions with enzyme in the presence of ssDNA and Mg2+. L, The quantitative data of the autoradiogram in K. M, Time dependence of ATPase activity of PfUDC1. The time of incubation in minutes is mentioned at the top of the autoradiogram and C is the control reaction without enzyme. N, The quantitative data of the autoradiogram in M.