Exploration of Lipid Metabolism in Relation with Plasma Membrane Properties of Duchenne Muscular Dystrophy Cells: Influence of L-Carnitine
Figure 1
L-carnitine related parameters in muscle cells. L-carnitine content, transport and OCTN2 mRNA levels were determined in control and DMD patient cell treated (or not) with 500 µM of L-carnitine.
Results are presented as histograms. Each histogram represented the means +/− sem of 7 independent determinations. Control cells were represented by white histogram, control cells treated with L-carnitine by light grey histogram, DMD cells by dark grey histogram and L-carnitine treated DMD cells by a black histogram. Statistical differences between samples are indicated by letters on top of the histograms. Two identical letters placed indicated a significant difference between the two samples (p<0.05). (A) L-carnitine content was determined in cultured muscle cells and L-carnitine content was expressed in nmol per mg of protein. (B) L-carnitine uptake was determined in cultured cells and expressed in fmol of L-carnitine transported per hour and per mg of protein. (C) OCTN2 mRNA levels were determined by RT-q-PCR. The amount was normalized and expressed relatively to control cells.