Cohesion Fatigue Explains Why Pharmacological Inhibition of the APC/C Induces a Spindle Checkpoint-Dependent Mitotic Arrest
Figure 7
APC/C activity is required for MCC disassembly independently of proteolysis.
(A) Timeline showing the experimental strategy. (B) Immunoblots of BubR1 immunocomplexes isolated from mitotic HeLa cells depleted of p31comet or Apc2 by RNAi, arrested in mitosis with nocodazole and released into media containing MG132, ZM447439 and AZ3146. Cyclin A was used as a marker for lack of APC/C activity. (C) Bar graph quantifying the experiment in (B). Values represent the mean ± s.e.m. of four independent experiments.