Cohesion Fatigue Explains Why Pharmacological Inhibition of the APC/C Induces a Spindle Checkpoint-Dependent Mitotic Arrest
Figure 5
Proteasome activity is not required for MCC disassembly.
(A) Timeline showing the experimental strategy. (B) Immunoblot of BubR1 immune complexes isolated from HeLa cells arrested in mitosis with nocodazole and released into media containing different combinations of MG132, ZM447439 and AZ3146. Cyclin B and phosphorylated histone H3 Ser10 (pH3) were used as markers for mitosis and Aurora B activity respectively. (C) Bar graph quantifying the experiment in (B). Values represent the mean ± s.e.m. of three independent experiments.