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Cross Regulation of Sirtuin 1, AMPK, and PPARγ in Conjugated Linoleic Acid Treated Adipocytes

Figure 5

PPARγ agonists or antagonists affect the TG loss caused by AMPK activators without t10c12 CLA being present.

a Differentiated 3T3-L1 adipocytes were incubated with or without 0.1 mmol/L phenformin (Phen), with or without PPARγ agonist troglitazone (Tro), and TG levels were measured after 24 h. b TG levels were measured in differentiated 3T3-L1 adipocytes in media lacking or containing 2 mmol/L metformin (Met), with or without PPARγ antagonist GW9662 (9662) for 24 h. Each bar represents the mean + SEM (n = 3), and is representative of three independent experiments. Means not sharing a common letter differ, P0.05.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0048874.g005