Localization of a Female-Specific Marker on the Chromosomes of the Brown Seaweed Saccharina japonica Using Fluorescence In Situ Hybridization
Figure 2
Southern blot of the labeled FRML-494 marker.
Ethidium-bromide-stained agarose gel (A) of NotI- (lanes 1 and 3) and XbaI- (lanes 2 and 4) digested genomic DNA from the female (lanes 1 and 2) and male (lanes 3 and 4) gametophytes of S. japonica. Southern blot (B) of NotI- (lanes 1 and 3) and XbaI- (lanes 2 and 4) digested genomic DNA hybridized with the biotin-labeled FRML-494 marker. Lanes M1 and M2 show the D2000 and 1 kb DNA ladder molecular standard, respectively, (Tiangen, Beijing, China).