Targeting 3-Phosphoinoside-Dependent Kinase-1 to Inhibit Insulin-Like Growth Factor-I Induced AKT and p70 S6 Kinase Activation in Breast Cancer Cells
Figure 4
PF-5177624 inhibited cell proliferation and cell transformation in MCF7 and T47D cells.
Cells were cultured in complete medium and treated with PF-5177624 for 72 hours prior to addition of rezasurin to determine the IC50 value of cell proliferation inhibition by PF-5177624 (A). Soft agar assays were performed in MCF7 (B and C) and T47D cells (D and E). The images from representative dishes and microscopy are shown to demonstrate colony numbers and morphology (B and D). Colony numbers were also counted (C and E).