Pleiotrophin (PTN) Expression and Function and in the Mouse Mammary Gland and Mammary Epithelial Cells
Figure 5
PTN effect on mammosphere formation.
(A) Time course of mammosphere formation day 1, 3 and 5 without and with a blocking anti-PTN antibody ± recombinant PTN protein. Representative mammospheres that meet the threshold surface area of >900 µm2 are indicated by an arrowhead. Pictures are representative of 3 independent experiments. Scale bar = 50 µm. (B) Quantification of mammospheres (>900 μm2 ) formed per field at day 5. Data are means ± SE; n = 5 fields/well; 4 wells for each condition. **P<0.01; versus anti-PTN treated cells; ***P<0.001 versus control by ANOVA. (C to F) Immunofluorescence of mammospheres at Day 5 for Ki67 (green)(C), Caspase 3 & DAPI (green & blue) (D), laminin & DAPI (green & blue) or for PTN (grey scale). Staining is shown in mammospheres treated with blocking anti-PTN antibody (anti-PTN) versus control. Scale bar = 20 µm.