A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure
Figure 5
The effect of pH on the fluorescence emission and absorbance of Rtms5Y67F, Rtms5Y67F/H146S, Rtms5 and Rtms5 H146S.
Absorbance and fluorescence emission spectra were determined at different pH in buffers of constant ionic strength for (A), Rtms5Y67F; (B), Rtms5Y67F/H146S; (C), Rtms5 and (D), Rtms5 H146S. Values shown are those at the and
for each protein. Excitation was 440 nm for A and B, and 590 nm for C and D.