Spermine Attenuates the Action of the DNA Intercalator, Actinomycin D, on DNA Binding and the Inhibition of Transcription and DNA Replication
Figure 6
Polyamine depletion enhances the inhibition of transcription by actinomycin D within cells.
The effects of ACTD at 3 µM on the fold change of c-myc gene expression in (A) HeLa, (B) A549, and (C) MCF7 cells by RT-PCR with and without a 24 h MGBG pretreatment versus control samples. β-actin was used as the internal control. The data represent the mean values ±SDs from three separate experiments (*p<0.05).