Spermine Attenuates the Action of the DNA Intercalator, Actinomycin D, on DNA Binding and the Inhibition of Transcription and DNA Replication
Figure 3
Effects of spermine on the stabilization of actinomycin D on DNA duplexes.
(A) Melting temperatures (Tm in °C) of DNA in the presence of spermine (SPM) at various concentrations without (filled column) or with (open column) ACTD. The DNA sequence was d(TTTGCAAA). (B) Gibb's free energy change, ΔG, for the formation of DNA duplexes in the presence of spermine (SPM) at various concentrations with (open column) or without (filled column) ACTD. Each value was averaged from three separate experimental sets.