Intrahepatic Infiltrating NK and CD8 T Cells Cause Liver Cell Death in Different Phases of Dengue Virus Infection
Figure 5
Intrahepatic as well as splenic CD8+ T cells recognize NS4B99–107 epitope.
(A) Liver lysates were collected from uninfected and infected wild type and STAT1 KO mice. Arrow points to cleaved caspase 3. Data presented are representative of three independent experiment (n = 3 per time point in each experiment). (B) Splenocytes were isolated from wild type and STAT1 KO mice at day 3 (empty), 5 (darkened), and 7 (hatched) after infection and at day 5 after UV-inactivated DENV injection (horizontal line). (C) IHLs were isolated from wild type and STAT1 KO mice at 5 days after DENV infection. Splenocytes and IHLs were stimulated with or without (control) indicated peptides. The percentages of IFNγ-producing CD8+ T cell within the total CD8+ T population were analyzed by flow cytometry. Data presented are representative of four independent experiments (n = 3 per time point in each experiment). ** P value <0.01 compared with the control group.