Intrahepatic Infiltrating NK and CD8 T Cells Cause Liver Cell Death in Different Phases of Dengue Virus Infection
Figure 4
Intrahepatic T cells are cytotoxic against DENV-infected hepatocytes.
(A) Liver lysates were collected from uninfected and infected wild type and TCRβ KO mice. Arrow points to cleaved capase 3. Data presented are representative of three independent experiments (n = 3 per time point in each experiment). (B) Uninfected (Uninf) or infected (Inf) Hepa 1–6 were co-cultured with IHLs isolated from uninfected (Uninf) or infected (Inf) mice at different ratio. Data presented are representative of three independent experiments (n = 3 per time point in each experiment). (C) Liver cryosections from infected mice at day 5 with or without anti-CD4 and anti-CD8 antibody were stained with TUNEL reagents. Data presented are representative of three independent experiments (n = 3 per time point in each experiment).