Intrahepatic Infiltrating NK and CD8 T Cells Cause Liver Cell Death in Different Phases of Dengue Virus Infection
Figure 2
Intrahepatic infiltration of NK and T cells coincides with CXCL10 and CCL5 productions in the liver.
(A) The levels of CXCL10 and CCL5 in sera collected from uninfected (Un) and infected mice were determined by ELISA. Data presented are from three independent experiments (n = 3 per time point in each experiment). (B) CXCL10 and CCL5 mRNAs in the liver were analyzed by RT-real-time PCR and normalized against GAPDH. Data presented are from three independent experiments (n = 3 per time point in each experiment). (C) Hepa 1–6 cells were infected with DENV at MOI of 1 and CXCL10 and CCL5 mRNA expressions were analyzed by RT-real-time PCR and normalized against GAPDH. Data presented are from three independent experiments (n = 2 per time point in each experiment). (D) IHLs were collected from uninfected (open) or infected mice at days 1 (shaded bar) and 5 (darkened) after infection. The percentage in total IHL population (left) and the absolute number (right) of CD3-CD49b+ NK cells, CD3+CD8+ and CD3+CD4+ T cells were analyzed by flow cytometry (n = 5 per time point in each experiment). * P value <0.05, ** P value <0.01 compared with uninfected groups.