Intrahepatic Infiltrating NK and CD8 T Cells Cause Liver Cell Death in Different Phases of Dengue Virus Infection
Figure 1
DENV administered intravenously causes transient viremia, liver cell infection and apoptosis.
Viral capsid gene expression in the serum of uninfected mice (Un) or mice at different time points after infection of DENV (d.p.i., days after infection) was determined by RT real-time PCR (n = 2 per time point in each experiment). (B) C6/36 cells were cultured in medium containing serum collected from uninfected or infected mice. Viral capsid gene expression was determined by RT-PCR. Beta-actin of C6/36 cells was used as internal control (top). Viral antigen expression was determined by rabbit anti-DENV antibody staining (bottom). Shaded histograms outlined by thin line and open histogram outlined by thick line represent cells cultured in serum from uninfected and infected mice respectively. Data presented are representative of two independent experiments (n = 3 per time point in each experiment). (C) Liver was collected from uninfected (Un) and infected mice. Viral capsid gene expressions were determined by RT-PCR. Data presented are representative of two independent experiments (n = 5 per time point in each experiment). (D and E) Liver was collected from uninfected (Un), mice infected with DENV and mice inoculated with otherwise equivalent UV-inactivated DENV (UV-DENV). Arrow points to cleaved caspase 3. Data presented are representative of five independent experiments (n = 3 per time point in each experiment).