Src Homology-2 Domain-Containing Protein Tyrosine Phosphatase (SHP) 2 and p38 Regulate the Expression of Chemokine CXCL8 in Human Astrocytes
Figure 5
Effect of SHP2 overexpression and p38 inhibition on CXCL8 levels.
SHP2WT- and SHP2CS-transfected astrocytes were treated with PTP inhibitor, Na3VO4; p38 inhibitor, SB203580, or negative control inhibitor, SB202474. CXCL8 levels measured by ELISA in cellular supernatants collected 24 h post-treatment with A) Na3VO4 B) SB203580 C) SB202474. ND denotes not detectable. Results are representative of three independent experiments performed in triplicate and expressed as mean ± SEM, analyzed by one-way ANOVA and Newman-Keuls post-test for multiple comparisons or student’s t-test.