Src Homology-2 Domain-Containing Protein Tyrosine Phosphatase (SHP) 2 and p38 Regulate the Expression of Chemokine CXCL8 in Human Astrocytes
Figure 2
Increased CXCL8 expression in astrocytes activated by IL-1β and TNF-α.
Cultured human astrocytes were treated with IL-1β (20 ng/ml) or TNF-α (20 ng/ml). A) CXCL8 mRNA expression 8 h post-activation normalized to GAPDH determined by real-time PCR. B) CXCL8 protein levels 24 h post-activation normalized to MTT determined by ELISA. Results are representative of three independent experiments performed in triplicates. Results are expressed as mean ± SEM, analyzed by one-way ANOVA and Newman-Keuls post-test for multiple comparisons.