Insulin-Like Growth Factor-I E-Peptide Activity Is Dependent on the IGF-I Receptor
Figure 2
EA and EB increase MAPK signaling in C2C12 cells.
A. Cells were starved in media without serum, and treated with synthetic E-peptides at concentrations indicated for 20 minutes. Protein lysates were separated via SDS-PAGE and immunoblotted for Phosphorylated ERK 1 and 2 (P-ERK1/2), stripped, and blotted for Total ERK 1 and 2 (T-ERK1/2). B–C. Quantification of A. D. Cells were treated as above at optimal doses (EA and Scr 1 µM, EB 10 nM) for times indicated. E–F Quantification of C. NoTx at 30 minutes was included in each experiment for normalization between blots. For B–C and E–F, bars represent means ± s.e.m. of N = 3 replicates. *, p<0.05; ***, p<0.001, for comparisons to NoTx via 2-way ANOVA followed by a Bonferroni post-test.