Hedgehog Pathway Signaling Regulates Human Colon Carcinoma HT-29 Epithelial Cell Line Apoptosis and Cytokine Secretion
Figure 5
Survival and proliferative activity of HT-29 cells upon exposure to different stimuli. HT-29 cell viability was analyzed at different time points with different treatments, using the MTT assay.
When HT-29 cells were exposed to cyclopamine, cell viability decreased significantly comparing 48 with 24 hours (*P<0.05) (A). For the purpose of analyzing changes in the proliferative activity of HT-29 cells upon different treatments, the cellular incorporation of BrdU was measured at 48 and 72 hours. After 72 hours, BrdU incorporation was maintained in vehicle-treated cells (DMSO), while it significantly decreased in all other treatment groups (*P<0.04). Within the group of cyclopamine exposed HT-29 cells, BrdU incorporation decreased significantly from 48 to 72 hours (**P<0.05) (B). Data are expressed as the mean ± SEM of 3 independent experiments.