Hedgehog Pathway Signaling Regulates Human Colon Carcinoma HT-29 Epithelial Cell Line Apoptosis and Cytokine Secretion
Figure 4
Monocyte chemotaxis induced by cell culture supernatants of HT-29 cells upon exposure to different stimuli.
Monocytes were incubated in the upper chambers of a 5 µm-pore-size polycarbonate filter in a transwell system, with the 24 h-culture supernatants of HT-29 cells treated with rShh, cyclopamine, LPS, or IFN-γ in the lower chambers. Supernatants of HT-29 untreated cells were used as controls and values were arbitrarily normalized to 1. Additional controls were obtained by the incubation with the chemoattractant MCP-1, and anti-MCP-1 antibody, as positive and negative controls, respectively. *Cell migration is significantly lower in cells treated with Shh compared to controls (P = 0.01), and those treated with GANT61 (P = 0.005), cyclopamine (P = 0.003), LPS (P = 0.042), IFN-γ (P = 0.017), and MCP-1 (P = 0.001), respectively. **Anti-MCP-1 significantly decreased chemokinesis compared with GANT61, cyclopamine, LPS, IFN-γ, or MCP-1 (P<0.02),. Data are expressed as the mean ± SEM of 3 independent experiments.