Hedgehog Pathway Signaling Regulates Human Colon Carcinoma HT-29 Epithelial Cell Line Apoptosis and Cytokine Secretion
Figure 3
Production of cytokines by HT-29 cell cultures upon exposure to different stimuli.
Concentration of cytokines in supernatants of 24 hour cultures of HT-29 cells measured by ELISA. Histograms show the levels of IL-8 (A) and MCP-1 (B), under various conditions: treatment with Shh (Sonic Hedgehog), Purm (purmorphamine), Butyrate, LPS, IFN-γ, EGF, with or without the addition of cyclopamine, GANT 61, or DMSO (vehicle control) for 24 hours. IL-8 levels are significantly lower in cells treated with either Shh, purmorphamine, or butyrate compared to those treated with cyclopamine (*P<0.02), GANT 61 (#P<0.04), or LPS (**P<0.03), respectively (A). MCP-1 levels are significantly lower in cells treated with Shh, purmorphamine, or butyrate compared to the ones treated with GANT 61 (#P<0.02), LPS (*P<0.03) IFN-γ (**P<0.03), or EGF (***P<0.04), respectively (B). Data are expressed as the mean ± SEM of 6 independent experiments.