Perlecan Domain V Induces VEGf Secretion in Brain Endothelial Cells through Integrin α5β1 and ERK-Dependent Signaling Pathways
Figure 2
DV increases both Akt and ERK phosphorylation.
(A) Immunoblots (upper) and OD quantification (lower) for phosphorylated Akt (pAkt) and pan-Akt (Akt) from BECs in the presence of DV and/or 10 µM LY294002 (PI3K inhibitor). N = 3, *P<0.05 and **P<0.01 versus 0 min timepoint, ##P<0.01 versus DV-only treated group (vehicle) (B) Immunoblots (upper) and OD quantification (lower) for pAkt and Akt in BECs treated with 10 µM AktIV (Akt inhibitor). N = 3, *P<0.05 and **P<0.01 versus 0 min timepoint. (C) Immunoblots (upper) and OD quantification (lower) for phosphorylated ERK (pERK) and pan-ERK (ERK) in BECs treated with 10 µM LY294002. N = 3, **P<0.01 versus 0 min timepoint, #P<0.01 versus DV-only treated group (vehicle). (D) Immunoblots (upper) and OD quantification (lower) for phosphorylated ERK (pERK) and pan-ERK (ERK) in BECs treated with 10 µM AktIV. N = 3, **P<0.01 versus 0 min timepoint, #P<0.01 versus DV-only treated group (vehicle).