Modelling Neuroinflammation In Vitro: A Tool to Test the Potential Neuroprotective Effect of Anti-Inflammatory Agents
Figure 5
Pro-inflammatory response in BV2 and primary microglia cell cultures in response to LPS/IFN-γ.
BV2 cells were treated 100 ng/mL of LPS +0.5 ng/mL of IFN-γ and primary microglial cells with 100 ng/mL LPS +30 ng/mL IFN-γ, and different pro-inflammatory parameters were measured. TNF-α (A) and IL-6 (B) levels were determined in the culture medium 24 h after treatment. COX-2 (C) and iNOS protein expression (D) were measured by western blot 12 h after treatment, and data normalized by β-actin. NO production (E) was determined in the culture medium 24 h after treatment. Bars are means + SEM of four to six independent experiments. *p<0.05 and **p<0.01 vs control; paired Student’s t test. (F) shows representative western blots.