Modelling Neuroinflammation In Vitro: A Tool to Test the Potential Neuroprotective Effect of Anti-Inflammatory Agents
Figure 4
High extracellular K+ concentration did not potentiate reactive glia-induced neurotoxicity in neuron-primary microglia co-cultures.
Neuronal viability (MAP2-ABTS-ELISA assay) in neuron-primary microglia co-cultures grown in 5 mM and 25 mM KCl and treated with 100 ng/mL LPS +30 ng/mL IFN-γ for 24 h. Results are presented as % of MAP2 immunostaining in control co-cultures grown in 5 mM KCl. Bars are means + SEM of four independent experiments. **p<0.01 vs each control; two-way ANOVA (repeated measures) and Bonferroni post-test.