Modelling Neuroinflammation In Vitro: A Tool to Test the Potential Neuroprotective Effect of Anti-Inflammatory Agents
Figure 3
Glial activation and resulting neurotoxicity in neuron-primary microglia co-cultures.
(A) Neuronal viability (MAP2-ABTS-ELISA assay) in neuronal cultures and neuron-primary microglia co-cultures 48 h after treatment with 100 ng/mL LPS and increasing concentrations of IFN-γ (15 and 30 ng/mL). (B) The addition of microglial cells at a microglia:neuron ratio of 1∶2 did not result in neurotoxicity after 48 h. Results are presented as % of MAP2 immunostaining vs each control. Bars are means + SEM of 3–4 independent experiments. *p<0.05 vs control; one-way ANOVA and Newman-Keuls post-test. MAP-2 immunocytochemistry in neuronal cultures (C-D) and neuron-primary microglia co-cultures (E-F) in control conditions (C and E) and 48 h after 100 ng/mL LPS +30 ng/mL IFN-γ (D and F).