The Dual Orexin/Hypocretin Receptor Antagonist, Almorexant, in the Ventral Tegmental Area Attenuates Ethanol Self-Administration
Figure 3
Almorexant inhibits intracellular calcium release in HEK-293 cells expressing either Ox-R1 or Ox-R2.
A. Activation of intracellular calcium release in HEK-293 cells expressing human Ox-R1 by orexin-A (1 pM-10 µM) (closed squares). Almorexant alone did not stimulate intracellular calcium release in Ox-R1 expressing cells (open triangles). B. Activation of intracellular calcium release in HEK-293 cells expressing human Ox-R2 by orexin-B (1 fM- 10 µM, closed squares). Almorexant alone did not stimulate intracellular calcium release in Ox-R2 expressing cells (open triangles). C. Inhibition of orexin-A (100 nM, closed squares) induced stimulation calcium release in Ox-R1 by SB-334867 (closed triangles) or almorexant (open triangles) (10 pM-100 µM). D. Inhibition of orexin-B (10 nM; closed square) induced stimulation of calcium release in Ox-R2 by SB-334867 (closed triangles) or almorexant (10 pM-100 µM, open triangles). Results are expressed as mean ± SEM relative fluorescence units (RFU) calculated as agonist-induced maximum calcium peak/cell number ×1000.