SU5416, a VEGF Receptor Inhibitor and Ligand of the AHR, Represents a New Alternative for Immunomodulation
Figure 3
In Vitro dose response curves. A.
Rat hepatoma cell lines bearing the murine AHRd (AHRd-15) or the rat AHRb (5L) were incubated with increasing doses of TCDD in 96-well plates. After 40 hours, EROD activity was performed from whole cell lysate. (n = 3). B. Same assay as in A, with increasing doses of SU5416. (n = 3). C. Same assay as in A, with increasing doses of BNF. (n = 2). D. Male DBA/2J and C57BL/6J mice (6 weeks old) were orally administered 120 mg/kg BNF, the indicated doses of SU5416 or the vehicle corn oil. Following 48 hours, hepatic microsomal proteins were isolated and used for the assessment of EROD activity. Animals were treated in groups of 4. Error bars: S.D.