Salinomycin Induces Autophagy in Colon and Breast Cancer Cells with Concomitant Generation of Reactive Oxygen Species
Figure 5
Partial protection against salinomycin toxicity through inhibition of autophagy.
Cell death was measured in SW620 by flow cytometry using the ViaCount reagent 24 hours after treatment with 10 µM salinomycin, or solvent control. (A) Cells were transfected with siRNA against ATG7, or control oligos, and treated with salinomycin 72 hours after transfection. (B) Protection against cell death induction by salinomycin after 3 hours pre-treatment with 1 µM Wortmannin.