Salinomycin Induces Autophagy in Colon and Breast Cancer Cells with Concomitant Generation of Reactive Oxygen Species
Figure 1
Effect of salinomycin on cell viability in colon and breast cancer cell lines.
The cell viability was determined by MTT and colony forming assays. (A) Colon cancer cell lines, 72 hours post treatment (p<0.001, unifactorial ANOVA); (B) breast cancer cell lines, 72 hours post treatment (p<0.001, unifactorial ANOVA). (C) Cell viability assay performed 6 days post treatment. Colon and breast cancer cell lines were treated with the indicated concentrations of salinomycin. Salinomycin reduced the cell viability in a time and concentration dependent manner (p<0.001, unifactorial ANOVA). (D) Sensitivity to salinomycin in the colony forming assay. Assays were performed in triplicates. After incubation for 10 days, the plates were stained with crystal violet. The reduction of colony number depended on the salinomycin concentration and the cell line (*: p<0.05; **: p<0.01 by unifactorial anova).