An Economical Method for Production of 2H,13CH3-Threonine for Solution NMR Studies of Large Protein Complexes: Application to the 670 kDa Proteasome
Figure 2
Scheme used for the biosynthesis of U-[2H],Thr-γ2[13CH3], 6.
Enzyme name abbreviations: KHGA = 2-keto-4-hydroxyglutarate aldolase, AAT = aspartate aminotransferase, HSK = homoserine kinase, TS = threonine synthase, BCAT = branched-chain-amino-acid aminotransferase.