An Economical Method for Production of 2H,13CH3-Threonine for Solution NMR Studies of Large Protein Complexes: Application to the 670 kDa Proteasome
Figure 1
Metabolism of Thr starting from Asp.
The structure of Thr is highlighted in the box and the Thr to Asp pathway is along the vertical. Also shown are pathways that potentially can be exploited to synthesize Thr and the enzymes that are involved (horizontal). Dashed lines (bottom) indicate the non-physiological conversion of 2-ketobutyrate into L-2-aminobutyrate and then into Thr.