Novel Levamisole Derivative Induces Extrinsic Pathway of Apoptosis in Cancer Cells and Inhibits Tumor Progression in Mice
Figure 5
Comparison of effect of 4a and Levamisole on progression of solid tumor in mice.
Solid tumor was induced in Swiss albino mice by injecting EAC cells. Six doses of 4a and Levamisole (20 mg/kg) each administered to tumor bearing mice on every alternate day from 12th day of EAC cell injection. A. Effect of 4a and Levamisole on tumor progression at different time points. Data shown is based on two independent batches of experiments containing four animals each. Error bars indicate SD from independent experiments. B. Kaplan–Meier survival curves of mice treated with 4a. Out of 24 tumor induced Swiss Albino animals, 12 were treated with 4a (20 mg/kg) and survival graph was plotted, Log-rank statistical test showed P<0.005 (**). In control case, median survival time was found to be 59 days and in case of 4a treated it is undefined (value showed up to 250 days). C. Gross appearance of 4a treated and untreated tumor mice and their selected organs at 25th day of treatment. a. mouse with no tumor, b. mouse bearing tumor, c. tumor bearing mouse after treatment with 4a, d. thigh tissue of normal mouse, e. tumor, f. thigh tissue of a treated mouse, g. liver from normal mouse, h. liver of a tumor mouse, i. liver from a 4a treated mouse, j. spleen of a normal mouse, k. spleen of a mouse with tumor, l. spleen of a treated mouse.