Mapping the Mechanome of Live Stem Cells Using a Novel Method to Measure Local Strain Fields In Situ at the Fluid-Cell Interface
Figure 5
Experimental and computational analysis of flow regimes around live stem cells.
(A) 2D micrographs of μ-PIV experiments show flow regimes in the apical and basal regions of the live stem cell (target shear stress imparted by flow: 0.2 dyn/cm2). (B,C) Validated computational fluid dynamics (CFD) predictions of velocity gradients (which drive shear gradients and hence target shear stresses) in the apical and basal regions of cells, at flow rates including 0.2 and 1 dyn/cm2, respectively. * indicates statistical significance, defined by p<0.05. Error bars show standard errors (n = 3).