A Small Molecule Agonist of EphA2 Receptor Tyrosine Kinase Inhibits Tumor Cell Migration In Vitro and Prostate Cancer Metastasis In Vivo
Figure 5
Doxazosin treatment causes EphA2 receptor internalization and induces cell rounding.
(A) Immunofluorescence staining of U373-A2 cells for EphA2 receptor (red) after treatment for 60 minutes with 50 µM DZ in 0.2% DMSO. Treatment with 1 µg/ml ephrin-A1-Fc and DMSO served as positive and negative controls, respectively. DAPI nuclear staining is shown in blue. (B) Immunofluorescence staining of MDA-MB-231 cells for EphA2 receptor (red) after treatment for 120 minutes with 50 µM DZ in 0.2% DMSO. Controls are as given above. Scale bars, 25 µm. (C) Images from cell rounding analysis of PC-3 cells stimulated with 50 µM doxazosin for 30 or 60 min. Stimulation with 2 µg/ml ephrin-A1-Fc for 10 min or 0.2% DMSO served as positive and negative controls, respectively. Cells were seeded on 6-well plates and stimulated after 24 hours.