Acyl Chains of Phospholipase D Transphosphatidylation Products in Arabidopsis Cells: A Study Using Multiple Reaction Monitoring Mass Spectrometry
Figure 6
Discriminant analysis of molecular species profiles according to lipid class and membrane fraction.
PE, PC, PI and PG profiles were analyzed by MRM mass spectrometry in different membrane fractions. The number of repetitions n for each phospholipid class and for each membrane fraction is indicated in Figure S6G. The profiles were used for a discriminant analysis. The score plot represents 86% of the total variability of molecular species profiles. F1/F2 are the 2 principal eigenvalues for this variability. The Variables (phospholipid molecular species)/Factors (F1 and F2) correlations are shown in the loading plot (insert). The score for each profile was calculated. For clarity, only the centroid corresponding to one phospholipid class in a specific membrane is shown. Each class associates a specific phospholipid (PC, PE, PI, PG) with the extract prepared from a sub-cellular membrane fraction. The full image where all scores are presented is in Figure S6E. Abbreviations: mit,mitochondria; n, nucleus; g, Golgi apparatus; m, microsomes; h, homogenate; d, DRM; r, RE; to, tonoplast; p, plasma membrane.