Acyl Chains of Phospholipase D Transphosphatidylation Products in Arabidopsis Cells: A Study Using Multiple Reaction Monitoring Mass Spectrometry
Figure 3
PLD is activated upon SA stimulation.
Cell medium was supplemented with 0.1% (v/v) n-butanol and forty-five minutes later SA was added. Lipids were extracted at different times after cells were treated with 750 µM SA (A) or at 80 min after the addition of SA at different concentrations (B). Lipids were analyzed by mass spectrometry in the MRM mode, searching for the transitions listed in Table 1. The sum of the areas of the peaks corresponding to transitions for PBut was normalized to the area of the peak corresponding to the transition corresponding to 14∶0/14∶0-PC or to 14∶0/14∶0-PG. The levels of PBut are expressed as percentage of that in the control.