Acyl Chains of Phospholipase D Transphosphatidylation Products in Arabidopsis Cells: A Study Using Multiple Reaction Monitoring Mass Spectrometry
Figure 1
Mass scan of the main glycerophospholipids in suspension cells.
Mass spectra of parent ions of phosphatidylethanolamine (A), phosphatidylcholine (B) and phosphatidylinositol (C) were recorded by the neutral loss of fragment 141 amu (from positive ions), the neutral loss of fragment 60 amu (from negative ions) and the precursors of fragment m/z 241 (from negative ions), respectively. Mass spectra correspond to the [M+H]+ ions for PE, [M+HCOO]− for PC and [M−H]− ions for PI. The glycerophospholipid fatty acid composition was determined in the negative mode by CID as shown (insert) for 16∶0/18∶2-molecular species.