Neisseria gonorrhoeae Suppresses Dendritic Cell-Induced, Antigen-Dependent CD4 T Cell Proliferation
Figure 3
Soluble factors in BMDC/T cell co-culture partially inhibit OVA-induced T cell proliferation.
A–C) CFSE proliferation profiles for OT-II T cells co-cultured with BMDCs under indicated conditions. Representative CFSE profiles for T cells from transwell insert (gray) and transwell itself (open) are shown (from three independent experiments). D) IL-10 protein production by BMDCs cultured with Medium, OVA, N. gonorrhoeae (MOI = 1, 1). Mean pg/mL ± SD, N = 3. E) Il12a, Il12b, Il23a and Ebi3 mRNA steady-state expression in BMDCs cultured with OVA, N. gonorrhoeae (MOI = 1), or N. gonorrhoeae (MOI = 1) with OVA. Mean fold regulation ± SD, N = 3. F) Steady-state expression of mRNA encoding TGF-β 1, 2 and 3 in BMDCs cultured with OVA, N. gonorrhoeae (MOI = 1), or N. gonorrhoeae (MOI = 1) plus OVA. Mean fold regulation ± SD, N = 3. G) Aldh1a2 mRNA steady-state expression in BMDCs cultured with OVA, N. gonorrhoeae (MOI = 1), or N. gonorrhoeae (MOI = 1) with OVA. Mean fold regulation (decrease) ± SD, N = 3.