14-3-3θ is a Binding Partner of Rat Eag1 Potassium Channels
Figure 10
Estimation of the single channel conductance of rEag1 channel under different co-expression paradigms.
rEag1 K+ channels were co-expressed with either the control vector, difopein, or 14-3-3θ in HEK293 cells. (A) Comparison of the mean current density (pA/pF) at +40 mV: rEag1+vector, 111.2±9.5; rEag1+difopein, 126.9±13.2; rEag1+14-3-3θ, 76.8±5.8. The numbers in the parentheses refer to the number of cells analyzed, and the asterisk denotes significant difference from the rEag1+vector control (*, t-test: p<0.05). (B) Representative non-stationary fluctuation analysis of rEag1 K+ currents. (Top row) Whole cell current traces were evoked by 300-ms depolarizations from −90 mV to +40 mV, which were used to generate the ensemble mean (second row) and variance (third row). (Bottom row) The mean-variance plot (during the 300-ms test pulse, with the linear capacitative component eliminated)(open circles) was fit with a parabolic function (solid curve) to estimate the single channel current (0.9 pA). (C) Box plot presentation of the distribution of the estimated single channel conductance. Mean values (pS): rEag1+vector, 7.5±0.9; rEag1+difopein, 7.0±2.3; rEag1+14-3-3θ, 8.0±1.1. No significant difference was found among the three co-expression conditions (One-way ANOVA: p>0.05).