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14-3-3θ is a Binding Partner of Rat Eag1 Potassium Channels

Figure 3

The contribution of PAS and CNBHD to rEag1 interaction with 14-3-3θ.

GST pull-down assays of rEag1 N-terminal and C-terminal GST fusion proteins containing specific structural domains. (Upper panels) Schematic representation of the rEag1 N-terminal (A) or C-terminal (B,C) GST fusion proteins. (Lower left panels) Coomassie blue staining of the GST proteins. (Lower right panels) Immunoblotting of pull-down products with the anti-myc antibody.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0041203.g003