Phevalin (aureusimine B)Production by Staphylococcus aureus Biofilm and Impacts on Human Keratinocyte Gene Expression
Figure 4
Increased amounts of phevalin did not impact the extracellular metabolome of S. aureus as detected by HPLC-MS.
(A) Overlays of representative HPLC-MS base-peak chromatograms of +PCM (red) and –PCM (blue) revealed no substantial differences with the exception of phevalin (arrow). (B) XCMS analysis identified 22 features significantly (p<0.05) regulated ±2 fold in +PCM relative to –PCM. Those features grouped by RT into four groups (numbered bars). Group 2 contained phevalin, its fragment ions, and various adducts. Groups 1, 3, and 4 contained trace features associated with compounds used during the synthesis of phevalin. Data represent means ± SEM, n = 3.