Long-Lasting LTP Requires Neither Repeated Trains for Its Induction Nor Protein Synthesis for Its Development
Figure 4
Lack of effect of anisomycin and cycloheximide, two translational inhibitors of protein synthesis, on L-LTP.
In the two parts of the figure (A, B), either drug was applied starting 20 min before the induction of LTP on the S1 pathway till the end of the experiment. LTP was induced using a single high-frequency stimulation train (100 Hz, 1 s). In each part of the figure, the time courses of the fEPSP slope in the S1 pathway and S2 pathway (control), both in the presence and in the absence of the protein-synthesis inhibitor used, are displayed. Sample fEPSP traces from individual experiments are shown in the insert; they were recorded in the presence of the drug, before (thin traces) and 8 h30 after (thick traces) LTP induction. (A) Lack of effect of anisomycin at a dosage of 40 µM. (B) Lack of effect of cycloheximide at a dosage of 300 µM.