Long-Lasting LTP Requires Neither Repeated Trains for Its Induction Nor Protein Synthesis for Its Development
Figure 3
Cycloheximide and anisomycin inhibit protein synthesis in hippocampal slices.
(A1–A2) Mice hippocampal slices were incubated in an interface chamber and CHX (300 µM), anisomycin (25 µM) or emetine (20 µM) was added for 85 min. [3H]-leucine (1 µCi/ml) was introduced for 1 hour during (A1) or 4 h after (A2) protein synthesis inhibitor application. We calculated the percentage of inhibition at each time point by determining the ratio between the TCA-precipitable radioactivity of each treated sample (set of 7 individual slices) and that of the corresponding control sample. (A3) Percentage of inhibition of protein synthesis during anisomycin (ANISO), cycloheximide (CHX) and emetine application and 4 h after ANISO or CHX application. (B1–2) Percentage of [3H]-leucine incorporation in slices submitted to forskolin and IBMX during 15 minutes. This percentage was calculated by determining the ratio between TCA-precipitable radioactivity of treated sample and that of the corresponding control sample.