A New Antifibrotic Target of Ac-SDKP: Inhibition of Myofibroblast Differentiation in Rat Lung with Silicosis
Figure 8
The RAS signal and Ac-SDKP in plasma and lung of rat silicotic model.
Expression of ACE, Ang II and AT1 was significantly increased in the silicotic model (4w and 8w) as compared to control (4w and 8w). Ac-SDKP post- or pre-treatment showed a pattern to decrease the silicosis-induced expression of ACE, Ang II, and AT1, but only inhibition of AT1 expression was statistically significant. A) The plasma level of Ang II measured by ELISA; C4: control 4w group; S4: silicotic model 4w group; C8: control 8w group; S8: silicotic model 8w group; Ac-post: Ac-SDKP post-treatment group; Ac-pre: Ac-SDKP pre-treatment group; B) The level of Ac-SDKP in the lung was measured by ELISA; C) The tissue extracts were analyzed by Western blot analysis with antibodies against ACE, Ang II and AT1; D) The ACE protein expression measured by Western blot. E) The Ang II protein expression measured by Western blot. F) The AT1 protein expression measured by Western blot.