Antibacterial Activity and Mechanism of a Scorpion Venom Peptide Derivative In Vitro and In Vivo
Figure 4
Secondary structure and enzyme release assay of Kn2-7.
(A) and (B) Secondary structure analysis of the Kn2-7 and BmKn2 peptides. (A) Circular dichroism spectra of 0.1 mg/mL of Kn2-7 in water, 30% TFE/H2O or 70% TFE/H2O. (B) Circular dichroism spectra of 0.1 mg/mL of BmKn2 in water, 30% TFE/H2O or 70% TFE/H2O. (C) and (D) Enzyme release assay. (C) S. aureus AB94004 treated with Kn2-7 or BmKn2 was harvested, and the catalase activities of the supernatants were measured; 0.9% saline was used as the negative control, and ampicillin sodium was used as the antibiotic control. (D) E. coli AB94012 treated with Kn2-7 or BmKn2 was harvested, and the catalase activities in the supernatants were measured; 0.9% saline was used as the negative control, and kanamycin was used as the antibiotic control.