On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase
Figure 9
Prolycopene isomerization mediated by CRTI in the presence of 2H2O.
A, HPLC analysis (HPLC system 4) showing the isomerization of tetra-cis-lycopene (prolycopene, peak 1) into tri-cis-lycopene (peak 2) and 7,9 di-cis-lycopene (peak 3) after a 3 h incubation (37°C) in the presence of 30 µg CRTI and 100 µM FADred under anaerobic conditions. B, mass spectra (numbering according to A) of remaining substrate and of products.