On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase
Figure 8
CRTI switch from desaturase to isomerase activity.
Trace a, showing the elution profile (HPLC system 2) of prolycopene (1) extracted from the liposomes used. Trace b, profile of a 3 h incubation (37°C) of prolycopene in the presence of 30 µg CRTI and 100 µM FADred under anaerobic conditions. A tri-cis-lycopene species (2) forms predominantly. Trace c, tri-cis-lycopene was purified and incorporated into liposomes. Trace d, liposomes from c, analyzed after incubation with CRTISO and FADred [11]. This shows the formation of 7,9-di-cis-lycopene (3), all-trans-lycopene (4) and 5-cis-lycopene (5). Trace e, profile of the iodine-catalyzed isomer equilibrium obtained with the tri-cis species in organic solution. UV/VIS spectra of relevant cis-lycopene species are displayed on the right panels.