On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase
Figure 4
Characterization of CRTI phytoene desaturase activity.
A, Dependence on the FAD concentration at a constant 7.5 µM phytoene concentration; B, variation of the phytoene concentration at a constant 150 µM FAD concentration. Phytoene liposomes were supplemented providing 0.8, 1.65, 2.89, 3.61, 5.57, 9.2 µM phytoene. Incubation time was 12 min at 37°C; C, Time course of lycopene formation in a standard incubation assay. Symbols represent the data from the mean of three replicate experiments (bars: ± SE). The curves through the data points in A and B are fits obtained with the Graphpad Prism software and using the equation Y = VmaxX/(Km+X).