On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase
Figure 3
CRTI phytoene desaturation activity.
A, standard incubation assay (see Experimental Procedures) extracted with CHCl3/MeOH 2∶1 (v/v) after an incubation time of 30 min. The yellow color in the aqueous epiphase is FAD. The organic phase contains the colorless phytoene substrate in the control (c, no CRTI added) or the red-colored lycopene (+15 µg CRTI). B, HPLC separation (system 1) of the organic phases shown in A (MaxPlot; peaks recorded at individual λmax). Insets show the corresponding UV/VIS spectra of phytoene (1) and all-trans-lycopene (2).