On the Structure and Function of the Phytoene Desaturase CRTI from Pantoea ananatis, a Membrane-Peripheral and FAD-Dependent Oxidase/Isomerase
Figure 2
SDS-PAGE analysis of overexpressed CRTI protein: fractions and purification.
The expected molecular mass of the overexpressed protein is 56 kDa. Lane M, molecular mass markers; lane 1, whole cell lysate after IPTG induction; lane 2, pellet after 12,000 × g centrifugation; lane 3, supernatant after 12,000 × g centrifugation; lane 4, fraction after IMAC purification; lane 5, fraction after GPC-purification.